CyFlow™ Beta2-MG Biotin

Antigen: | β2-microglobulin |
Clone: | B2M-01 |
Application: | Flow cytometry |
Format/Fluorochrome: | Biotin |
Target Species: | Human |
Field of Interest: | Blood group antigens, MHC |
Species of Origin: | Mouse |
Regulatory Status: | RUO |
Clonality: | monoclonal |
Isotype: | IgG2a |
Concentration Unit | mg/mL |
Concentration | 1 |
Quantity | 0.1 mg |
Volume | 0.1 |
Immunogen | Purified human β2-microglobulin |
Background Information | β2-microglobulin (β2M) non-covalently associates with the 44 kDa α chain to forms the HLA Class I antigen complex. Human β2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. |
Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
| Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M‑01 for detection and purification of human beta 2‑microglobulin. Folia·Biol·(Praha). 1984; 30(6):369‑76. <·PMID:·6394369·> | Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA‑G Expression in Human Neural Cells after Neurotropic Viral Infections. J·Virol. 2005·Dec; 79(24):15226‑15237. <·PMID:·16306594·> | Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr·Nephrol. 2006·Sep; 21(9):1257‑65. <·PMID:·16810512·> | Poláková K, Železníková T, Russ G: HLA‑G5 in the blood of leukemia patients and healthy individuals. Leuk·Res. 2013·Feb; 37(2):139‑45. <·PMID:·23146557·>
Available Technical Data Sheets
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