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CyStain UV Precise P

CyStain UV Precise P
Fluorescent probe: DAPI
Application: Flow cytometry
Laser UV LED / UV laser
Quantity 250 tests
Emission Maximum 461 nm
Order number: 05-5002

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

  • 05-5002
Ready-to-use reagent kit to determine ploidy level with a two-step protocol. Using the kit... more
CyStain UV Precise P

Ready-to-use reagent kit to determine ploidy level with a two-step protocol. Using the kit components for nuclear extraction and staining of nuclear DNA from plant tissues, genome size variations and ploidy levels can be determined. The reagent has been created specifically for selected configurations of the Sysmex CyFlow® Ploidy Analyser and CyFlow® Space flow cytometers.

Kit components:

  • 125 mL Nuclei Extraction Buffer
  • 500 mL Staining Buffer
Specific References

Atri C. & Banga S.S. A protocol for flow cytometric determination of expected chromosome number of Brassica juncea L. introgression lines. Journal of Oilseed Brassica (2014); 5 (2): 170-174.
De K.K.; Saha A.; Tamang R. & Sharma, B. Investigation on relative genome sizes and ploidy levels of Darjeeling-Himalayan Rhododendron species using flow cytometer. Indian Journal of Biotechnology (2010); 9: 64-68.
Matsuda M.; Iwata Y.; Koizumi N. & Mishiba K.I. DNA double-strand breaks promote endoreduplication in radish cotyledon. Plant Cell Reports 2018; 37(6): 913-921. https://doi.org/10.1007/s00299-018-2278-5.
Nasution O.; Sitorus A.C.; Nelson S.P.C.; Forster B.P. & Caligari P.D.S. A high-throughput flow cytometry method for ploidy determination in oil palm. Journal of Oil Palm Research (2013); 25 (2): 265-271.
Padoan D.; Mossad A.; Chiancone B.; Germanà M.A. & Sha Valli Khan P.S. Ploidy levels in Citrus clementine affects leaf morphology, stomatal density and water content. Theoretical and Experimental Plant Physiology (2013); 25 (4): 283-290.
Thammina C.S.; Olsen R.T.; Kramer M. & Pooler M.R. Genetic relationships of boxwood (Buxus L.) accessions based on genic simple sequence repeat markers. Genet Resour Crop Evol (2017); 64: 1281–1293. doi: 10.1007/s10722-016-0436-6
Wolny E.; Betekhtin A.; Rojek M.; Braszewska-Zalewska A.; Lusinska J. & Hasterok R. Germination and the early stages of seedling development in Brachypodium distachyon. Int J Mol Sci. (2018 Oct); 19(10): 2916. doi: [10.3390/ijms19102916]